Research Paper
Exosomal miR-15b-5p derived from gastric cancer cells enhances immune infiltrating of tumor-associated macrophages through the WIF1/WNT5A axis
Figure 1. Gastric cancer cells promoted the formation of tumor-associated macrophages and enhanced the miR-15b-5p expression in tumor-associated macrophages. THP-1 monocytes were co-cultured with GC cells for 24 hours to obtain TAMs. (A) QRT-PCR was adopted to calculate the expression of molecular markers (MMP1, MMP2, MMP9, IL-4, IL-10, and VEGF) of TAMs. (B) WB was implemented to verify the expression of molecular markers (iNOS, CD68, CD163 and CD20) of TAMs. (C) The miR-15b-5p level was monitored by qRT-PCR. (D) The WIF1/WNT5A expression was examined by WB. *P<0.05, **P<0.01, ***P<0.001 (vs. con group), N=3.