Research Paper Volume 13, Issue 11 pp 14843—14861

Figure 5. YY1 directly promotes TP73-AS1 promoter activation. (A) The indicated promoter activity was measured in SHY5Y cells over expressing YY1 or empty vector. * p<0.05 in the two-tailed t-test; NS, not significant. (B) CRISPRi SHY5Y cells were treated with DOX for 10 days to induce YY1 KD. Cells were treated with TMZ (750 μM for 48 hours). The activity of TP73-AS1-promoter-824 was measured using the dual luciferase assay. * p<0.05 in the two-tailed t-test; NS, not significant. (C) Schematic representation of the WT or mutant YY1 binding sites within the TP73-AS1 promoter luciferase construct. (D) SHY5Y cells expressing WT or mutant promoter were treated with TMZ or DMSO control for 48 hours after which promoter activity was measured using dual luciferase assay. * p<0.05 in the two-tailed t-test; NS, not significant.