Research Paper Volume 13, Issue 14 pp 18191—18222

Figure 2. MiR-545-3p and miR-195 were sponged and modified by lncRNA AFAP1-AS1 in triple-negative breast cancer (TNBC) cells. (A, B) Expressions of miR-545-3p (A) and miR-195 (B) were lower in MDA-MB-231 and BT-549 cell lines than in MCF-10A cell line. *: P<0.05. (C, D) Expressions of miR-545-3p (C) and miR-195 (D) were boosted in MDA-MB-231 and BT-549 cell lines after respective transfections of pcDNA6.2/GW/EmGFP-miR-545 and pcDNA6.2/GW/EmGFP-miR-195. *: P<0.05. (E, F) MiR-545-3p (E) and miR-195 (F) were sponged by lncRNA AFAP1-AS1 in certain targets, and MDA-MB-231 and BT-549 cell lines of pmirGLO-WT-lncRNA AFAP1-AS1+pcDNA6.2/GW/EmGFP-miR-545/miR-195 group were associated with weaker luciferase activity than TNBC cell lines of pmirGLO-MUT-lncRNA AFAP1-AS1+pcDNA6.2/GW/EmGFP-miR-545/miR-195 group. *: P<0.05. (G) LncRNA AFAP1-AS1 expression in MDA-MB-231 and BT-549 cell lines was determined when pcDNA3.1-lncRNA AFAP1-AS1 was transfected. *: P<0.05. (H, I) Expressions of miR-545 (H) and miR-195 (I) were detected among MDA-MB-231 and BT-549 cell lines transfected by pcDNA3.1, pcDNA3.1-lncRNA AFAP1-AS1, si-NC and si-lncRNA AFAP1-AS1. *: P<0.05.