Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 13, Issue 11 pp 15285—15306
Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer’s disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway
Figure 2. BM-MSC-EVs have therapeutic effects on AD hippocampal neurons. The AD neuron model was established by Aβ1-42 induction, and then AD neurons were treated with BM-MSC-EVs or equal volume of BM-MSC conditioned medium after GW4869 treatment. (A) Immunofluorescence assay was used to detect Aβ content in hippocampal neurons. (B) ELISA was used to detect level of Aβ1-42; (C) MTT assay was used to detect the viability of AD hippocampal neurons; (D) Flow cytometry was used to detect the apoptosis rate of AD hippocampal neurons. The experiment was repeated three times, and the data was expressed as mean ± standard deviation. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. **p < 0.01, ***p < 0.001.