Research Paper Volume 13, Issue 11 pp 14968—14988
Construction of a novel mRNA-miRNA-lncRNA network and identification of potential regulatory axis associated with prognosis in colorectal cancer liver metastases
Figure 8. Expression of ZEB1-AS1, miR-335-5p and APOC1 in CRC tumor tissues and APOC1 promotes CRC cell migration and invasion in vitro. (A–C) Expression of APOC1, ZEB1-AS1 and miR-335-5p in normal colon tissues and CRC tumor tissues with metastasis or without metastasis were detected by RT-qPCR. (D) APOC1 expression was knocked down successfully using small interference RNA (siRNA) in HCT116 cells, and APOC1 expression was overexpressed using a plasmid vector in SW480 cells. All expression levels were detected by western blotting. Representative immunoblots and the ratios of the indicated proteins to GAPDH are presented. (E, F) Knockdown of APOC1 expression inhibited HCT116 cells migration and invasion, while overexpression of APOC1 increased SW480 cells migration and invasion (Scale bar: 100 μm). (G) The novel mRNA-miRNA-lncRNA competing endogenous RNA (ceRNA) triple regulatory sub-network related to prognosis of CRLM. ns (Not significant), *p < 0.05, **p < 0.01, ****p < 0.0001.