Research Paper Volume 13, Issue 11 pp 14968—14988
Construction of a novel mRNA-miRNA-lncRNA network and identification of potential regulatory axis associated with prognosis in colorectal cancer liver metastases
Figure 7. ZEB1-AS1 regulates APOC1 expression by competing for miR-335-5p to promote CRC cells invasion and migration. (A–C) miR-335-5p inhibitor abolished effects of ZEB1-AS1 downregulation on APOC1 expression in HCT116 and SW480 cells. All expression levels were detected by western blotting and RT-qPCR. Representative immunoblots and the ratios of the indicated proteins to GAPDH are presented. (D–F) ZEB1-AS1 overexpression eliminated the effects of miR-335-5p mimics on APOC1 expression in HCT116 and SW480 cells. All expression levels were detected by western blotting and RT-qPCR. Representative immunoblots and the ratios of the indicated proteins to GAPDH are presented. (G, H) miR-335-5p inhibitor rescued the effects of ZEB1-AS1 downregulation on HCT116 cell invasion and migration, and ZEB1-AS1 overexpression rescued the effects of miR-335-5p mimics on SW480 cell invasion and migration (Scale bar: 100 μm). *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001.