Research Paper Volume 13, Issue 10 pp 14198—14218

Figure 1. LOC146880 expression is upregulated in ESCC tissues and cells. (A) The expression levels of LOC146880 in 21 pairs of ESCC and adjacent normal esophageal tissues. (B) Kaplan-Meier survival curve analysis shows overall survival of ESCC patients with high- and low-expression levels of LOC146880 (n = 81). (C) QRT-PCR analysis shows LOC146880 expression levels in human esophageal epithelial cells and ESCC cell lines (EC109, TE-1, Kyse30, Kyse70, Kyse150, and Kyse410). (D) FISH analysis shows LOC146880 expression in ESCC and normal esophageal tissues. (E) Bioinformatics analysis (https://genome.ucsc.edu/) shows H3K27ac levels in the promoter region of LOC146880. (F) ChIP assay analysis with anti- H3K27ac antibody shows H3K27ac levels in the LOC146880 promoter of HEEC, Kyse30, and TE-1 cells. (G) ChIP assay analysis with anti-CBP antibody shows CBP protein levels in the LOC146880 promoter of HEEC, Kyse30, and TE-1 cells. (H) Western blot analysis shows CBP protein levels in control and CBP-silenced Kyse30, and TE-1 cells. (I) ChIP assay analysis shows H3K27ac levels in the LOC146880 promoter of control and CBP-silenced Kyse30, and TE-1 cells. (J) QRT-PCR analysis shows LOC146880 expression levels in control and CBP-silenced Kyse30 and TE-1 cells. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.