Research Paper Volume 13, Issue 12 pp 15964—15989

Figure 1. Antrodia salmonea (AS) inhibits the growth and induces intracellular ROS generation in human colon cancer cells. (A–C) SW620, HCT116, and HT-29 cells were treated with AS (0–300 μg/mL) for 24 h. Cell viability was determined using the MTT assay. (D, E) Cells were treated with 200 μg/mL AS for 0–120 min. 10 μM of DCFH₂-DA was mixed in the culture medium 30 min before of each experiment and then intracellular ROS levels were measured and expressed in graph as a fold of the control. Each value is expressed as the mean ± SD (n = 3) and significant at ^*p < 0.05; ^**p < 0.01; and ^***p < 0.001 when compared with control.