Research Paper Volume 13, Issue 9 pp 13138—13152

Figure 8. CircITGA7 functioned as a sponge of miR-34a-5p and VEGFA was a target of miR-34a-5p in glioma. (A) The binding site of miR-34a-5p in circITGA7. (B) Relative expression of miR-34a-5p in glioma tissues and paired adjacent normal tissues. (C) SW1783 and U373 were transfected with wide-type or mutant circITGA7 reporter plasmid, and the luciferase reporter was performed to confirm the direct target site. (D) SW1783 and U373 cells were transfected with wide-type or mutant VEGFA reporter plasmid, and the luciferase reporter was performed to confirm the direct target sites. (E) Overexpression of miR-34a-5p inhibited the levels of VEGFA in SW1783 and U373 cells by qRT-PCR. (F) Western blot results showed that the level of VEGFA protein in cells transfected with si-circITGA7 decreased, while the level of VEGFA protein in cells transfected with miR-34a-5p inhibitor increased, compared with the control group, miR-34a-5p inhibitor can reduce si-circITGA7 decreases the level of VEGFA protein. GAPDH was as the control. (G) The miR-34a-5p inhibitor eliminated the inhibitory effect of circITGA7 knockdown on VEGFA in SW1783 and U373 cells. *p < 0.05, **p < 0.01, ***p<0.001.