Figure 3. Interleukin-8 receptor A (IL8-RA) immunohistochemical staining to determine the formation and width of the inflammatory belt and density of neutrophils. (A) IL8-RA immunohistochemical staining of skin tissues. (B) IL8-RA immunohistochemical staining of subcutaneous tissues. (C) Comparation of inflammatory belt formation, inflammatory belt width, and density of neutrophils. The blue circle indicates abnormal neutrophil aggregation under the dermis of diabetic rat. *: P<0.05, **: P<0.01, ***: P<0.001.