Research Paper Volume 13, Issue 8 pp 11969—11987

Figure 5. miR-580-5p up-regulate the CCL2 expression by decrease the expression of PPARα. (A and B) Huh-7 and HepG2 cells were co-transfected with miR-580-5p mimic or inhibitor and hsa_circ-0110102 expression vector or vector control for 48 h. CCL2 mRNA and protein levels were detected with RT-qPCR and Western blot analysis. (C) Predicted binding sites of miR-580-5p in the 3′ UTR of PPARα. (D) Huh-7 and HepG2 cells were transfected with miR-580-5p mimic or inhibitor for 48 h, the protein levels of PPARα were detected with western blot assay. (E) Huh-7 and HepG2 cells were treated with 5 μM WY-14643 or 10 μM GW4671 for 48 h, the protein and mRNA levels of CCL2 were detected with western blot and RT-qPCR. Data are presented as mean ± standard error. *, P < 0.05.