Research Paper Volume 13, Issue 7 pp 10749—10769
Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer
Figure 4. MiRNA-150 triggers the activation of the Wnt and Hh signaling pathways. (A) MiRNA-150 activated TOPFlash/FOPFlash luciferase activity. A TOPFlash/FOPFlash assay was conducted in BGC-823 cells transfected with miRNA-150 inhibitors. (B) The expression of β-catenin in the cytoplasm and nuclei of BGC-823 cells transfected with miRNA-150 inhibitors is shown. (C) Immunofluorescence analysis of the localization of β-catenin in BGC-823 cells transfected with miRNA-150 inhibitors is shown. (D, E) MiRNA-150 activated Wnt and Hh target gene expression. The expression of Wnt and Hh downstream target genes in BGC-823 and HFE-145 cells was detected by western blotting. (F) MiRNA-150 induced EMT. The expression of EMT markers in BGC-823 cells or HFE-145 cells was measured by western blotting.