Research Paper Volume 13, Issue 10 pp 13626—13643

Figure 7. Effects of E2F2 expression levels on GC cell migration and invasion via autophagy mediation. (A) Western blotting analysis of P62 and LC3-II protein expression in GC cells transfected with GV141-Vector or GV141-E2F2. β-actin was used as a loading control. (B) Western blotting analysis of P62 and LC3-II protein expression in GC cells transfected with siNC or siE2F2. β-actin was used as a loading control. (C) Representative electron micrographs of autophagic vesicles in GC cells transfected with GV141-Vector or GV141-E2F2 and in GC cells transfected with siNC or siE2F2. (D) GC cells were transfected with GV141-Vector or GV141-E2F2 for 24 h. Comparison of GC cell migration and invasion using Transwell compartments. (E) A wound-healing assay was performed to compare the motility of GC cells. The wound-healing area was analyzed using ImageJ software. (F) GC cells were transfected with siNC and siE2F2 or treated with phosphate-buffered saline (control), 3-methyladenine (2 mM) or a combination of both treatments for 24 h. Comparison of GC cell migration and invasion using Transwell compartments. (G) Wound-healing assay was performed to compare the motility of GC cells. (H) Western blotting analysis of P62, Beclin1, LC3-II and MMP9 protein expression. β-actin was used as a loading control. Data are presented as the mean ± S.D. from three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.