Research Paper Volume 13, Issue 8 pp 11705—11726
Selenomethionine protects hematopoietic stem/progenitor cells against cobalt nanoparticles by stimulating antioxidant actions and DNA repair functions
Figure 6. Suppression of DNA damage response signal attenuated the protection of SeMet against CoNPs. An inhibitor of ATM/ATR (CGK733) was added to CD34+ HSC/HPCs treated with SeMet and CoNPs. Afterwards, cells underwent cell viability (A) and apoptosis (B) assays. BRCA1 and UBE2K were silenced in CD34+ HSC/HPCs, followed by cell viability (C), apoptosis (D), cell cycle (E) and western blot assays (F). ***p < 0.001 vs. control cells that did no subjected to any treatments. ##p < 0.01 and ###p < 0.001 vs. cells treated with CoNPs alone. &p < 0.05, &&p < 0.01 and &&&p < 0.001 vs. cells treated with SeMet and CoNPs in combination.