Research Paper Volume 13, Issue 8 pp 11629—11645

Figure 4. Hsa_circ_0072309 negatively regulated miR-607 via MRE. (A) Real-time PCR was performed to detect the expression of hsa-miR-207, hsa-miR-214-3p, hsa-miR-336-5p, hsa-miR-607 and hsa-miR-781 in hsa_circ_0072309-downregulated H1975 and H1650 cells. (B) The expression level of miR-607 in tumor and normal tissues (n = 20). (C) The negative correlation of hsa_circ_0072309 with miR-607. (D) Real-time qPCR shows the expression level of miR-607 in hsa_circ_0072309-KD H1975 and H1650 cells. (E) RNA pulldown analysis of hsa_circ_0072309 against miR-607. (F) RNA pulldown was performed to confirm the putative miR-607 binding sites with hsa_circ_0072309. (G) A schematic diagram showing the putative miR-607 binding sites with hsa_circ_0072309. Hsa_circ_0072309-WT and hsa_circ_0072309-Mut luciferase reporter vectors were cotransfected with miR-607 or miR-NC and then subjected to luciferase activity analyses. Quantitative results are indicated in the right panel. Two-way ANOVA. N.S.: none significance; ^*p < 0.05, ^**p < 0.01.