Research Paper Volume 13, Issue 8 pp 11433—11454

Figure 3. LncRNA and mRNA expression verified by real-time qPCR. (A) Real-time qPCR results for the upregulated lncRNAs CLYBL-AS1, LINC01021, G012825, GS1-600G8.5, and AC020594.5 in P5 chondrocytes compared to P1 cells from three individual donors. (B) Real-time qPCR results for the downregulated lncRNAs AP001505.9, RP6-65G23.3, LINC00473, LINC00162, and G062245 in P5 chondrocytes. (C) Real-time qPCR results for the upregulated mRNAs, AK5, GDF5, and VNN1 in P5 chondrocytes. (D) Real-time qPCR results for the downregulated mRNAs EGLN3, FGFBP2, MMP3, and COL9A1 in P5 chondrocytes. (E) Co-expression network analysis of lncRNAs and mRNAs; red circles represent lncRNAs, blue circles represent mRNAs, solid lines represent positive regulation, and dotted lines represent negative regulation. GAPDH and ACTB were used as the internal references. Data are represented as means ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant.