Research Paper Volume 13, Issue 8 pp 11234—11256
Ischemic-hypoxic preconditioning enhances the mitochondrial function recovery of transplanted olfactory mucosa mesenchymal stem cells via miR-181a signaling in ischemic stroke
Figure 2. Determination of optimal cultivation scheme of IhOM-MSCs. (A) We were pretreated OM-MSCs with ischemia/hypoxia at different time gradients, and the RT-qPCR result showed that miR-181a was significantly down-regulated after 36h. (B) Successful mimic of miR-181a in IhOM-MSCs was verified by qPCR. (C–E) Protein and mRNA expression of GRP78 and Bcl-2 in OM-MSCs as determined by western blot and qPCR, respectively. (OM-MSCs were replaced by MSCs in the figure. All data are presented as the mean value ± SD. *p<0.05, **p<0.01).