Figure 7. Carminic acid attenuates the pathological symptoms in the Fru-induced chronic renal injury. (A) H&E (Scale bar = 50 μm), PAS (Scale bar = 50 μm), Sirius red (blue arrow: collagen accumulation; Up panel, Scale bar = 100 μm; Down panel, Scale bar = 50 μm) and Masson’s trichrome (yellow arrow: collagen accumulation; Up panel, Scale bar = 100 μm; Down panel, Scale bar = 50 μm) staining of renal sections for the calculation of histological changes. (B) Glomerular area was quantified. Calculation of fibrosis condition following (C) Sirius red and (D) Masson’s trichrome staining. (E) RT-qPCR analysis for fibrotic genes in renal tissues. (F) Western blot analysis of podocin in kidney samples. The results are expressed as the means ± SEM. n = 4 in each group.*P< 0.05, **P< 0.01 and ***P< 0.001 compared with the Ctrl group; +P< 0.05 and ++P< 0.01 compared with the Fru group.