Figure 3. Effect of BCCAO microbiota on brain structure and function. (A) Virtual graphics show brain functional connectivity (FC) in micecontrol and miceBCCAO. (B) The mean functional connectivity strength per brain network. * denotes P < 0.05 compared with control mice using a two-way repeated-measures ANOVA with post-hoc Tukey multiple comparisons test. All values are expressed as means ± S.D; n=15. (C) The mean FC matrices show the strength of functional connectivity between pairs of brain regions in control and BCCAO-treated groups. The color scale represents the strength of functional connectivity. (D) Correlation analyses of 8 regions of interest (ROIs) in the mouse brain. The brain regions analyzed include orbitofrontal cortex (OC), cingulate cortex (CC), somatosensory cortex (SC), thalamus (T), hippocampus (HC), motor cortex (MC), auditory cortex (AC), and visual cortex (VC).