Figure 3. miR-512-5p targets JAG1. (A) Venn diagrams show the targets of miR-512-5p. (B) The predicted miR-512-5p binding site on JAG1 3'-UTR (wild type, WT) is shown in red. (C) Dual luciferase reporter assays were used to confirm the binding site of miR-512-5p and JAG1. ( n.s, no significance, *p < 0.05, **p < 0.01, Student's t-test, compared to the JAG1-WT group.) (D) JAG1 expression in NBTs (n = 8) and glioma tissues (n = 71) as determined by western blotting. (*p < 0.05, Student's t-test) (E) The relationship between miR-512-5p and JAG1 was performed by Pearson’s correlation test (r = -0.3352, represent inverse relationship). (F) JAG1 expression determined after transfecting cells with corresponding vectors by western blotting analysis. *p < 0.05, **p < 0.01, Student's t-test, compared to the Control group. Data are represented as the mean ± standard deviation of three independent experiments.