Research Paper Volume 13, Issue 7 pp 9900—9910

Figure 4. MNX1-AS1 sponges miR-370. (A) The MNX1-AS1 expression was examined in cytoplasm and nucleus of TU212 cells. U6 was used as the nuclear control and GAPDH was used as the cytoplasmic control. (B) The predicted miR-370 binding sites in the region of MNX1-AS1 and the corresponding mutant sequence were shown. (C) Effect of miR-370 on the luciferase activity of WT-MNX1-AS1 and MT-MNX1-AS1 reporter systems was detected via luciferase reporter assay. (D) RNA-Pull down assay was conducted to assess the relationship between miR-370 and MNX1-AS1. (E) The expression of miR-370 was examined in TU212 cells transfected with sh-MNX1-AS1 or sh-NC. (F) MNX1-AS1 expression was examined by qRT-PCR in TU212 cells transfected with miR-370 mimics, miR-NC, miR-370 inhibitor (anti-miR-370) and anti-miR-NC. (G) The expression of miR-370 was examined in LSCC tissues and adjacent normal tissues. (H) qRT-PCR analysis of the expression of miR-370 in human LSCC cell line TU-212 and normal bronchial epithelial cell line (16HBE). (I) Correlation between MNX1-AS1 and miR-370 expression in LSCC tissues was analyzed by Pearson’s correlation analysis. ^*P < 0.05; ^**P < 0.01.