Research Paper Volume 13, Issue 8 pp 11083—11095

Figure 6. (A) Bioinformatic prediction of miR-3182 target genes via three major miRNA databases (Targetscan, miRDB, DIANA). (B) Correlation of mRNA expression of KLF4 and expression level of miR-3182 in NSCLC lung cancer tissue samples. (C) Bioinformatic prediction on the binding sites of miR-3182 with wild-type KLF4 and mutated KLF4 3’- UTR. (D) Effects of miR-3182 mimics and inhibitors modulative effects on miR-3182 expression value; (E, F) Dual-luciferase reporter gene assay on wild-type or mutated KLF4 expression influenced by miR-3182 inhibitor or miR-3182 over-expression. (G) Western-Blot analysis on the protein expression level of KLF4 influenced by miR-3182 mimic or inhibitor. (H) RT-PCR analysis on the mRNA expression level of KLF4 influenced by miR-3182 mimic or inhibitor.