Research Paper Volume 13, Issue 7 pp 9874—9899

Figure 3. Overexpression of DNASE1L3 relieves cell senescence and SASP under DDR activation. (A) Representative images of SA-β-Gal staining of cells in differently treated groups (scale bar, 200 μm). (B) Statistics of SA-β-Gal staining cells in differently treated groups. (C, D) Remnant cell number were analyzed by cell counts enumerated from differently treated groups at various time points. (E) Transcriptional level of canonical SASP factors in differently treated groups, with values normalized to the vector control per factor. The heatmap for the mean RT-qPCR data is shown. (F) Immunoblot analysis of inducible expression change of senescence associated signal pathway and downstream proteins including p53, p65, SPINK1 and AREG in different treated groups. (G) Dual luciferase assay showed activated NF-kB signaling in different treated groups. (H, I) ELISA analysis of IL-6, IL-8 secretion in supernatants from cells in different treated groups.