Figure 5. Effects of miR-590-3p on CRC cell proliferation and cell cycle progression. HCT116 and SW620 cells were transfected with miR-590-3p, and (A) cell viability was examined by CCK-8 assay; (B) colony formation capacity was examined by colony formation assay; (C) cell cycle progression was examined by flow cytometry; (D) the protein levels of CDK1, cyclin B1, and cyclin A2 were examined by immunoblotting. **P < 0.01, compared to the miR-NC group.