Figure 3.The knockdown of OGFRP1 inhibited the migration in human gastric cancer cells. (A) The migrations of AGS and MKN45 cells with the transfection of control siRNA or OGFRP1 specific-siRNA were evaluated by transwell. (B) Statistical analysis of migration cell number was performed using the t-test. (C) Wound healing assay was performed to further confirm the migration of AGS and MKN45 cells. The healing speed of scratches was calculated by healing area after scratching for 48 h/ total scratch area. (D) Statistical analysis of AGS cells was performed using the t-test. (E) Statistical analysis of MKN45 cells was performed using the t-test. (F) The expressions of migration-related proteins were determined using western blot. Statistical analyses of protein expression levels in AGS (G) and MKN45 (H) cells were performed using the t-test. *P<0.05.
