Research Paper Volume 13, Issue 7 pp 9732—9747

Figure 4. Comprehensive analysis of microbiology, lipidomics and transcriptomics. (A) Lipidomics showed content of PC increase in normal mice of Rb1 group comparing with that of Model group. Y axis is shown in foldchange, X axis shows different electron cut-off points of PC. All the metabolites with VIP >1 and P <0.05.PC, phosphatidylcholine. POS, positive ion model. NEG, negative ion model. (B) RNA-seq and qRT-PCR showed expression of RNAs associated with glycerophospholipid metabolism. RNA-seq was performed once, PCR were repeated thrice, n=3, data was shown in mean +/- SD. (C) Serum level of hyperlipidemia associated index showed Rb1 reduced total cholesterol level. TG, triglycerides; TC: total cholesterol; LDL, low density lipoprotein; HDL, high density lipoprotein;** represents P<0.01, n=6. (D) Lipidomics showed content of PC increase in fecal transplanted germ-free mice of Rb1 group comparing with that of Model group. Y axis is shown in foldchange, X axis shows different electron cut-off points of PC. All the metabolites with VIP >1 and P <0.05.PC, phosphatidylcholine. (E) QRT-PCR showed that Pmet increased and Dgkg, Phospho1, Pla4g4b decreased in fecal transplanted germ-free mice of Rb1 group. This meant Rb1 increased synthesis and decelerate decomposition of phosphatidylcholine.