Figure 7. HMGCS2 regulates Wnt3a/β-catenin signal transduction and AMPK phosphorylation in HepG2 cells. Cultured HepG2 cells were transfected with Hmgcs2 siRNA or control siRNA, followed by incubation with high palmitate or oleate for 24 h. (A) Representative western blot for Wnt3a/β-catenin. (B, C) Quantification of Wnt3a/β-catenin protein levels relative to GAPDH from three different cultures. (D) Representative western blot for p-AMPK in mouse liver. (E) Quantification of p-AMPK protein levels relative to total AMPK. Data are presented as mean ± SD, n = 3. * P < 0.05 vs control + control siRNA and † P < 0.05 vs palmitate + control siRNA.