Figure 2. JB-3-22 treatment caused abnormal spindle assembly and misaligned chromosome in porcine oocytes. (A) Images of spindle morphologies and chromosome alignment in control and JB-3-22 treated oocytes, Scale bar, 5 μm. (B) The proportion of abnormal spindles was recorded in control and JB-3-22 treated oocytes. (C) The proportion of misaligned chromosomes was recorded in control and JB-3-22 treated oocytes. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. * P < 0.05 and ** P < 0.01.