Research Paper Volume 13, Issue 7 pp 9566—9581

Figure 3. miR-125a attenuates cell proliferation, migration invasion, the EMT process and induces apoptosis. KYSE-150 and KYSE-150R cells were transfected with miR-125a mimic or miR-125a inhibitor or their corresponding negative controls. (A) The cell proliferation assay was performed at the indicated time points. (B) Representative micrographs of cell migration assays (left) and the quantification (right). (C) Representative micrographs of cell invasion assays (left) and the quantification (right). (D) Representative micrographs of cell apoptosis assays (left) and the quantification (right). (E) Western blot analysis revealed that the E-cadherin expression level was elevated, while N-cadherin and Vimentin levels were decreased in cells transfected with miR-125a mimic. Data are shown as mean ± SD from three independent experiments. *P<0.05 by Student’s t-test.