Research Paper Volume 13, Issue 6 pp 8665—8687

MicroRNA-95-3p serves as a contributor to cisplatin resistance in human gastric cancer cells by targeting EMP1/PI3K/AKT signaling


Figure 6. Over-expression of EMP1 leads to decreased cell viability, weakened invasion and migration ability, and enhanced cellular apoptosis. (A) Transfection of EMP1 ov vectors increased expression of EMP1. (B) MTT assay demonstrated that overexpressed EMP1 induced weakened cell survival rate of DDP-resistant SGC7901 cells. (C) Result of the BrdU assay indicated that overexpressed EMP1 lowered cellular viability of DDP-resistant SGC7901. (D) Cellular apoptosis rate of DDP-resistant SGC7901 cells with different treatments. (E, F) Results from transwell assay demonstrated lower cell invasion and metastasis of DDP-resistant SGC7901 upon up-regulation of EMP1. (G) Wound healing assay indicated up-regulation of EMP1 leads to decreased invasive ability in DDP-resistant SGC7901. (H) Western blot assay helped examine the down-stream regulatory proteins of EMP1. ***p<0.001, **p<0.01 compared to NC group.