Research Paper Volume 13, Issue 6 pp 8643—8664
Follistatin-like protein 1 functions as a potential target of gene therapy in proliferative diabetic retinopathy
Figure 10. The expression of FSTL1, TGFβ, CTGF, VEGF, FN, COL1, and α-SMA proteins in HUVECs. (A) shows western blots representatives of FSTL1, TGFβ, CTGF, VEGF, FN, COL1, α-SMA, and β-actin in HUVECs. (B–H) shows the ratio of FSTL1, TGFβ, CTGF, VEGF, FN, COL1, and α-SMA to β-actin in the hypoxic treated HUVECs and normal controls, respectively. Indicates the quantitative analysis of western blots figures in the normal, 3 h hypoxia, and 6 h hypoxia models using ImageJ. The 3 h and 6 h hypoxia results indicated that FSTL1, TGFβ, CTGF, VEGF, FN, COL1, and α-SMA were expressed at significantly higher levels than in normal cells (N: normal, h3: 3 h hypoxia, h6: 6 h hypoxia). Data are expressed as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.