Figure 2. Alterations of C5aR and proinflammatory cytokines level. (A) The content of C5a in serum was measured by ELISA assay. (B) The protein expression of C5aR in lung tissues were detected by western blotting. (C, D) The concentrations of pro-inflammatory cytokines including IL-1β, IL-18, IL-6, TNF-α and HMGB1 in BALF (C) and the lung tissues (D) were measured by ELISA kits, respectively. n = 7. *P < 0.05, **P < 0.01 and ***P < 0.001.