Research Paper Volume 13, Issue 6 pp 8588—8598
Inhibition of complement C5a receptor protects lung cells and tissues against lipopolysaccharide-induced injury via blocking pyroptosis
Figure 2. Alterations of C5aR and proinflammatory cytokines level. (A) The content of C5a in serum was measured by ELISA assay. (B) The protein expression of C5aR in lung tissues were detected by western blotting. (C, D) The concentrations of pro-inflammatory cytokines including IL-1β, IL-18, IL-6, TNF-α and HMGB1 in BALF (C) and the lung tissues (D) were measured by ELISA kits, respectively. n = 7. *P < 0.05, **P < 0.01 and ***P < 0.001.