Research Paper Volume 13, Issue 5 pp 7627—7643
Nicotine exacerbates atherosclerosis through a macrophage-mediated endothelial injury pathway
Figure 4. Nicotine activates the pyroptosis pathway in BMDMs by upregulating ROS production. (A, B) Western blot was used to detect the expression of TXNIP, NLRP3, ASC, caspase-1, and GSDMD. The average ratios were calculated based on gray intensity analysis (*P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001, n = 4). (C) IL-1β and IL18 in the supernatant of BMDMs treated with or without nicotine were measured by ELISA (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n=5).