Research Paper Volume 13, Issue 8 pp 11026—11042
Calycosin stimulates the proliferation of endothelial cells, but not breast cancer cells, via a feedback loop involving RP11-65M17.3, BRIP1 and ERα
Figure 3. Calycosin regulated cell proliferation by activating or repressing the RP11-65M17.3-ERα loop. (A–E) HUVECs, HMEC-1 cells, MCF-7 cells and T47D cells were treated with calycosin (10, 20 and 40 μM) for 12, 24, or 48 h. Western blotting was used to determine the protein levels of ERα and BRIP1, and β-actin served as the internal control. (F, G) The levels of RP11-65M17.3 were determined by qRT-PCR and normalized to those of β-actin.