Research Paper Volume 13, Issue 5 pp 7517—7537

Figure 7. Abemaciclib substantially inhibited the proliferation of HCC cells in vitro. (A) The protein levels of retinoblastoma (p-S780) and retinoblastoma in Hep3B and Huh7 cells. (B, C) Clone formation assays were used to detect the effects of abemaciclib on the proliferation of Hep3B and Huh7 cells. (D) A CCK-8 assay was used to analyze cell proliferation in control and abemaciclib-treated Hep3B and Huh7 cells. (E) An Edu assay was used to analyze cell proliferation in control and abemaciclib-treated Hep3B and Huh7 cells. Bar= 100μm; For (C, D), * was compared with Control (**, P < 0.01; ***, P < 0.001).