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Research Paper Volume 13, Issue 5 pp 7067—7083

GIT1 protects traumatically injured spinal cord by prompting microvascular endothelial cells to clear myelin debris

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Figure 8. Overexpression of GIT1 increases myelin-induced autophagy in vitro. (AD) LC3, P62, and VEGF levels in BMECs from the control and GIT1-OE groups after being treated with or without myelin debris for 72 h. (E) BMECs from the control and GIT1-OE groups were transfected with mRFP-GFP-LC3 adenovirus. The cells nuclei were stained using DAPI (blue). Bar, 10 μm. (F) Numbers of yellow and red puncta. N = 5 per group. NS represents no significance, *p < 0.05, **p < 0.01, ***p < 0.001.