Resveratrol improves Gly-LDL-induced vascular endothelial cell apoptosis, inflammatory factor secretion and oxidative stress by regulating miR-142-3p and regulating SPRED2-mediated autophagy

Wenjun Sha; Meizhi Liu; Dusang Sun; Junhui Qiu; Bilin Xu; Lin Chen; Tian Shen; Cheng Chen; Hongping Wang; Cuiping Zhang; Tao Lei

doi:doi:10.18632/aging.202546

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Research Paper Volume 13, Issue 5 pp 6878—6889

Resveratrol improves Gly-LDL-induced vascular endothelial cell apoptosis, inflammatory factor secretion and oxidative stress by regulating miR-142-3p and regulating SPRED2-mediated autophagy

Figure 5. Inhibition of SPRED2 reverses the effect of resveratrol on Gly-LDL-induced HUVEC cell proliferation, apoptosis, inflammatory factor secretion and oxidative stress. (A, B) PCR result of overexpression of miR-142-3p and SPRED2. (C) Cell viability of each group was tested by MTT. (D) The expression of SPRED2 in all groups by western blotting. (E) The expression of inflammatory factors IL-6, TNF-α, VCAM- and VEGF. (F) The expression of oxidative stress factors MDA, SOD and ROS. (G) The expression of caspase-3, BAX and BCL-2 in all groups by western blotting. (H) Statistical analysis of Western blotting results. (I) The apoptosis of all groups was detected by flow cytometry, and statistical analysis of flow cytometry results. *, P < 0.05; **, P < 0.01; ***, P < 0.001.