Figure 4. Detection of autophagy level of GSCs treated with TMZ, CQ, or both. SU4 and SU5 cells were exposed to TMZ (400 μM), CQ (10 μM), or both, respectively for 48 h. Then p62 and LC3 II expression levels were analyzed by Western blotting (A). Statistical analysis of p62 and LC3 II expression in SU4 and SU5 cells (B–E). *p < 0.05, ***p<0.001.