Research Paper Volume 13, Issue 5 pp 6820—6831
Nicardipine sensitizes temozolomide by inhibiting autophagy and promoting cell apoptosis in glioma stem cells
Figure 3. Autophagy-related proteins expression in GSCs after treating with TMZ, nicardipine, or both. Western blot analysis of p62, LC3 II and mTOR in SU4 and SU5 cells treated with TMZ (400 μM), nicardipine (20 μM), or both, respectively for 48 h (A). Statistical analysis of p62, LC3 II and mTOR protein levels in SU4 and SU5 cells (B–G). The mCherry-GFP-LC3 distribution in GSCs cultured with TMZ, nicardipine or both after 48 h was analyzed by confocal microscopy. Scale bar 100 μM (H). * p <0.05, ** p <0.01, *** p <0.001.