Research Paper Volume 13, Issue 5 pp 6820—6831

Figure 2. Combining dosing of nicardipine and TMZ decreased cell viability and promoted apoptosis of GSCs. SU4 cell viability was assessed by CCK-8 assay under exposure to TMZ (400 μM), nicardipine (20 μM) or both for 48 h (A). SU4 cells were treated with TMZ (400 μM), nicardipine (20 μM) or both for 48 h, then were stained with PI and Annexin V-FITC for apoptosis analysis (B, C). SU5 cell viability was assessed by CCK-8 assay under treatment with TMZ (400 μM), nicardipine (20 μM) or both for 48 h (D). SU5 cells were treated with TMZ (400 μM), nicardipine (20 μM) or both for 48 h, then were stained with PI and Annexin V-FITC for apoptosis analysis (E, F). Western blot analysis of Bcl-2, Bax, mitochondrial Bax and COX IV in GSCs was performed after treatment with TMZ, nicardipine or both for 48 h (G–K). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.