Research Paper Volume 13, Issue 5 pp 6752—6764

Figure 1. miR-23a is upregulated in DRG neurons derived from mice after SNI. (A) miR-23a expression in contralateral L5 DRG neurons at 7 day after model establishment, normalized to U6 (Scale bar = 50 μm) and quantitative histogram of miR-23a + neurons using FISH; (B) miR-23a expression in DRG neurons of sham-operated and SNI mice were determined using RT-qPCR, normalized to U6; (C) macrophages in DRG (F4/80+ cells, red) and miR-23a (green) detected using FISH and immunohistochemistry (Scale bar = 50 μm); (D) miR-23a expression in DRG neurons treated with 0.001% DMSO in HEPES buffer + glucose (1 mg/mL) and with capsaicin (1 μM), normalized to U6. Values obtained from three independent experiments are expressed as mean ± SD and analyzed by unpaired t-test. * indicates p < 0.05. Cell experiment was independently repeated for three times.