Research Paper Volume 13, Issue 2 pp 3101—3111
Sleep deprivation aggravates brain injury after experimental subarachnoid hemorrhage via TLR4-MyD88 pathway
Figure 4. Sleep deprivation activates TLR4 signaling after SAH. (A) Immunofluorescence staining shows that sleep deprivation increased TLR4 expression in neurons after SAH. Scale bar, 50 μm. (B, C) RT-PCR show that sleep deprivation increased the mRNA levels of TLR4 (B) and MyD88 (C) after SAH. (D) Western blot shows that sleep deprivation increased the expression of TLR4 and MyD88 after SAH. The data was represented as means ± SEM. #p < 0.05 vs. Sham group, *p < 0.05 vs. SAH group.