Research Paper Volume 12, Issue 23 pp 24394—24423

Figure 2. FBXW7 knockout in myeloid cells remodels the tumor immune microenvironment by promoting M2-like TAM polarization. (A) Tumors were digested to obtain single-cell suspensions. Ly6G⁺CD11b⁺ myeloid-derived suppressor cells (MDSCs), Ly6C⁺CD11b⁺ MDSCs, and MHCII⁺CD11c⁺ dendritic cells in the tumors were analyzed by flow cytometry. (B) Statistical analysis of the results in (A) (n = 5 per group). (C, D) Flow cytometry analysis (C) and statistical analysis (D) of F4/80⁺CD11b⁺ macrophages and CD206⁺ macrophages in tumors (n = 5 per group). (E, F) Flow cytometry analysis (E) and statistical analysis (F) of CD3⁺ T cells infiltrating the tumor, CD4⁺ and CD8⁺ T cells in CD3⁺ T cells (n = 5 per group). (G) Relative mRNA expression of Arg1, Fizz1, Ym1, VEGFα, and MMP9 in tumors was measured by qRT-PCR (n = 6 per group). Data are shown as the mean ± SD and are representative of three independent experiments. *P < 0.05; ***P < 0.001; ****P < 0.0001; n.s, no significance (Student’s t test (B, D, F, G)).