Research Paper Volume 13, Issue 2 pp 2251—2263

Figure 7. ARRB2 protects hepatocytes against IR injury by activating the PI3K/Akt pathway. L02 cells were treated with H₂O₂ to mimic oxidative stress during ischemia reperfusion. (A) Overexpress of ARRB2 marked increases in PI3K and Akt phosphorylation and PCNA expression as well as notably decreased cleaved caspase-3 levels. (B) Knockdown of ARRB2 significantly inhibited PI3K/Akt phosphorylation, inhibited PCNA expression and increased cleaved caspase-3 expression. (C) PI3K/Akt inhibitor PX866 significantly inhibited ARRB2 dinduced-PI3K/Akt phosphorylation, downregulated PCNA expression and upregulated cleaved caspase-3 expression. The data are presented as the Mean ± SD, n = 3. P<0.05, P< 0.01, P< 0.001using Student’s-t test.