Research Paper Volume 13, Issue 2 pp 2118—2134

Figure 4. IGF2 improves the mitochondrial functional activity of oocytes from aged mice. (A) Mitochondria were stained with mitotracker Green FM (green). Scale bar = 20 μm. (B) Quantification of mitochondrial distribution signals in control oocytes (n = 26) and IGF2-treated oocytes (n = 25). A Student’s t-test (two-tailed). *p < 0.05. Error bars indicate the SEM. (C) JC-1 staining showing the mitochondrial membrane potential (MMP) in control and IGF2-treated oocytes. (D) Quantification of the red/green fluorescence intensity ratio in control oocytes (n = 40) and IGF2-treated oocytes (n = 35). A Student’s t-test (two-tailed). *p < 0.05. Error bars indicate the SEM. (E) HPG Fluorescent staining showing total protein synthesis in MII-stage oocytes with or without IGF2-treatment. Oocytes were incubated in M16 medium with 50 μM HPG for 1 h prior to staining. Scale bar = 30 μm. (F) Quantification of HPG signal intensity in control (n = 28) and IGF2-treated (n = 29) oocytes. *p < 0.05. A Student’s t-test (two-tailed). Error bars indicate the SEM.