Research Paper Volume 12, Issue 23 pp 23996—24008

Figure 3. Overexpression of hsa_circ_0048179 increases GPX4 levels in HepG2 cells. (A) HepG2 cells were incubated with oleate/palmitate (2:1 molar ratio) for 24 h, after which levels of hsa_circ_0048179 were detected using qRT-PCR. (B) HepG2 cells were transfected with NC or lenti-hsa_circ_0048179 for 72 h. Hsa_circ_0048179 levels in HepG2 cells were detected using qRT-PCR. (C) Levels of GPX4 expression in HepG2 cells were detected with western blotting. (D) Relative expression of GPX4 in HepG2 cells was quantified by normalization to GAPDH. **P<0.01 vs. control group.