Research Paper Volume 12, Issue 23 pp 23974—23995
Effect of lncRNA WT1-AS regulating WT1 on oxidative stress injury and apoptosis of neurons in Alzheimer's disease via inhibition of the miR-375/SIX4 axis
Figure 4. LncRNA WT1-AS/WT1 inhibited OSI and apoptosis by inhibiting miR-375 expression. (A) The expression of WT1-AS, WT1 and miR-375 detected by qRT-PCR. (B) The expression of p-Tau and total Tau detected by western blot. (C) Detection of mitochondrial membrane potential by JC-1 staining. (D) Detection of ATP content. (E) Detection of ROS content. (F) Detection of MDA content, SOD and GSH-Px activities. (G) Detection of LDH activity. (H) Detection of apoptosis by flow cytometry. *P<0.05; the experimental results are expressed as the mean ± standard deviation. Differences among multiple groups were analyzed using one-way ANOVA followed by Tukey’s post hoc test. The experiment was repeated three times.