Research Paper Volume 12, Issue 23 pp 23960—23973
The long noncoding RNA HCG18 participates in PM2.5-mediated vascular endothelial barrier dysfunction
Figure 4. The lncRNA HCG18/miR-21-5p/SOX7/VE-cadherin axis is regulated by PM2.5 in vascular endothelial cells. (A) The binding relationship of SOX7 and the promoter region of VE-cadherin was predicted online. (B) Dual-luciferase reporter assay showed that SOX7 promote the transcriptional activities of VE-cadherin. (C) The protein expression of VE-cadherin was up-regulated in SOX7-overexpressed HUVECs. (D) The quantitative analysis of the results of C. (E) Overexpression of HCG18 promoted the expression of VE-cadherin, and the induction effect was abrogated by both overexpression of HCG18 or SOX7 and treatment with PM2.5. (F) The quantitative analysis of the results of E. ns: no significantly different. ***: P<0.001.