Research Paper Volume 12, Issue 19 pp 19766—19784
Autism-like behaviors and abnormality of glucose metabolism in offspring derived from aging males with epigenetically modified sperm
Methylation changes in F0 aged sperm implicated alterations in several autism-associated and glucose metabolism associated pathways. (A) Whole genome bisulfite sequencing (WGBS) identified differentially methylated regions (DMRs) in F0 aged sperm (n=2), showing the distribution of DMRs (q<0.05) in comparison to F0 young sperm (n=3). (B) the pie plot showed the total number of DMRs within promoter regions. Red and green indicate hypermethylated and hypomethylated promoter regions in F0 aged sperm, respectively. (C) Gene ontology (GO) analysis of biological function identified significantly enriched terms in DMRs within whole genome. (D) KEGG analysis revealed significantly enriched pathways among the genes with DMRs in whole genome. Red and green bars indicate the hypermethylated and hypomethylated regions in F0 aged sperm, respectively.