Research Paper Volume 12, Issue 19 pp 19766—19784

Reduced body weight, impairment of glucose tolerance and insulin secretion in OF-F1 and VOF-F1 male, not female, offspring. (A) The body weight trajectories in male F1 offspring (n=30 male F1 offspring for each group, ≥ 5 litter size/group). (B) Fasting blood glucose. (n=15 male F1 offspring for each group, ≥ 5 litter size/group). (C–F) Blood glucose during glucose tolerance test (left panel) and AUC results (right panel) at 6 (C and D) and 8 (E and F) weeks of age (n=15/each sexual offspring, ≥ 5 litter size/group). (G) Plasma insulin concentration in glucose-stimulated conditions. (n=15/each sexual offspring, ≥ 5 litter size/group). YF-mF1, OF-mF1 and VOF1-mF1 were referred as to the F1 male offspring from young, old and very old F0 father, respectively, meanwhile YF-fF1, OF-fF1 and VOF1-fF1 were referred as to the F1 female offspring from young, old and very old F0 father. Data are presented as the mean ± s.e.m. NS, P≥0.05; *, P<0.05, ***, P<0.001, versus control l (linear regression analysis for GTT and insulin assays and one-way ANOVA for AUC results).